4LPY
Crystal structure of TENCON variant G10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-03-18 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 80.340, 80.340, 73.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.920 |
| R-factor | 0.19617 |
| Rwork | 0.194 |
| R-free | 0.23464 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tes |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.034 | 0.154 |
| Number of reflections | 19740 | |
| Completeness [%] | 92.3 | 52.8 |
| Redundancy | 4.1 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris, pH 8.5, 2.5 M sodium formate, 5% isopropanol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
