4LPM
Frog M-ferritin with magnesium, D127E mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 183.913, 183.913, 183.913 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.978 - 1.650 |
R-factor | 0.128 |
Rwork | 0.126 |
R-free | 0.16370 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.179 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.710 |
High resolution limit [Å] | 1.650 | 3.550 | 1.650 |
Rmerge | 0.081 | 0.036 | 0.376 |
Number of reflections | 32597 | ||
<I/σ(I)> | 10 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 20.8 | 19.8 | 20.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 2M MgCl2, 0.1M Bicine, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 9 |