4LPL
Structure of CBM32-1 from a family 31 glycoside hydrolase from Clostridium perfringens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 93.15 |
Detector technology | CCD |
Collection date | 2010-07-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.033 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 55.620, 55.671, 88.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.637 - 1.350 |
R-factor | 0.1888 |
Rwork | 0.188 |
R-free | 0.20730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2j1a |
RMSD bond length | 0.006 |
RMSD bond angle | 1.066 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.400 |
High resolution limit [Å] | 1.350 | 2.910 | 1.350 |
Rmerge | 0.063 | 0.044 | 0.449 |
Number of reflections | 30335 | ||
<I/σ(I)> | 28.9 | ||
Completeness [%] | 98.9 | 94.2 | 97.7 |
Redundancy | 11 | 5.8 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 32% PEG 4000, 225mM MgCl2 100mM Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |