4LPA
Crystal structure of a Cdc6 phosphopeptide in complex with Cks1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 84.690, 84.690, 239.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.147 - 2.900 |
| R-factor | 0.2485 |
| Rwork | 0.245 |
| R-free | 0.28140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qb3 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.388 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEIT |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.980 | 3.004 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.046 | 0.260 |
| Number of reflections | 20254 | |
| <I/σ(I)> | 12.65 | 3.57 |
| Completeness [%] | 99.8 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293.15 | 400mM potassium sodium tartrate, 0.1M MES, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
