4LP1
Crystal structure of CyaY protein from Psychromonas ingrahamii in complex with Eu(III)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-04-02 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.91907 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.821, 49.992, 46.580 |
Unit cell angles | 90.00, 90.43, 90.00 |
Refinement procedure
Resolution | 31.147 - 1.803 |
R-factor | 0.2158 |
Rwork | 0.213 |
R-free | 0.26020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hs5 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.235 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
Rmerge | 0.039 | 0.019 | 0.298 |
Number of reflections | 30293 | ||
<I/σ(I)> | 14.4 | ||
Completeness [%] | 91.7 | 83.3 | 89.8 |
Redundancy | 1.6 | 1.6 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 297 | 20mM sodium acetate, 200mM MgCl2, 29.5% PEG 4000, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 297K |