4LOW
Structure and identification of a pterin dehydratase-like protein as a RuBisCO assembly factor in the alpha-carboxysome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 41 |
| Unit cell lengths | 51.110, 51.110, 60.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.110 - 1.300 |
| R-factor | 0.2025 |
| Rwork | 0.203 |
| R-free | 0.20360 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXCD |
| Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 51.110 |
| High resolution limit [Å] | 1.300 |
| Number of reflections | 36178 |
| Completeness [%] | 96.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 17.5% PEG3350, 800 mM ammonium formate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
