4LOU
Structure of the E148Q mutant of CLC-ec1 deltaNC construct in the absence of halide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2013-07-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 231.617, 100.316, 170.955 |
| Unit cell angles | 90.00, 131.71, 90.00 |
Refinement procedure
| Resolution | 39.724 - 2.981 |
| R-factor | 0.2122 |
| Rwork | 0.210 |
| R-free | 0.25280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ene |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.268 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.050 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Rmerge | 0.121 | 0.744 |
| Number of reflections | 55897 | |
| <I/σ(I)> | 26.1 | 1.426 |
| Completeness [%] | 99.4 | 91.6 |
| Redundancy | 7.3 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 23% PEG 400, 20mM Na/K tartrate, 50mM Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
