4LO5
HA70-alpha2,3-SiaLC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97952 |
Spacegroup name | I 41 3 2 |
Unit cell lengths | 260.740, 260.740, 260.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.717 - 2.700 |
R-factor | 0.1906 |
Rwork | 0.190 |
R-free | 0.20800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.797 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.600 | 2.900 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.075 | 0.636 |
Number of reflections | 41439 | |
<I/σ(I)> | 12 | 2.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.1 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 4.4 | 292 | 0.1 M sodium acetate, 1.5 M ammonium chloride, pH 4.4, EVAPORATION, temperature 292.0K |