4LO1
HA17-HA33-Gal
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97921 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 107.131, 118.697, 162.449 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.169 - 2.254 |
R-factor | 0.1999 |
Rwork | 0.199 |
R-free | 0.22740 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.768 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.900 | 2.400 |
High resolution limit [Å] | 2.254 | 2.254 |
Rmerge | 0.054 | 0.603 |
Number of reflections | 48977 | |
<I/σ(I)> | 14.5 | 1.9 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 3.6 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.2 | 292 | 0.1 M MES, 0.1 M magnesium chloride, 5% PEG8000, pH 6.2, EVAPORATION, temperature 292.0K |