4LNZ

Crystal structure of human Myosin 5b globular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.978
Spacegroup name	P 21 21 2
Unit cell lengths	60.644, 78.442, 86.606
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.139 - 3.110
Rwork	0.253
R-free	0.31240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	1.772
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	PHENIX (1.8.3_1479)

Data quality characteristics

	Overall
Low resolution limit [Å]	58.140
High resolution limit [Å]	3.100
Number of reflections	7647
Completeness [%]	96.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	50 mM Tris pH 7.0, 0.2 M succidic acid, VAPOR DIFFUSION, HANGING DROP, temperature 291K