4LNT

Crystal Structure of tRNA Proline (CGG) Bound to Codon CCC-U on the Ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-02
Detector	PILATUS 6M-F
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	209.811, 449.414, 620.861
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	155.215 - 2.940
R-factor	0.221046047
Rwork	0.219
R-free	0.26453
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	1.208
Data scaling software	XSCALE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	181.782		2.940
High resolution limit [Å]	2.870	12.840	2.870
Rmerge	0.162	0.014	0.014
Number of reflections	1302800	14695	96695
<I/σ(I)>	8.53	36.42	1.06
Completeness [%]	98.7	93.8	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl2, pH 7.0, vapor diffusion, temperature 293K