4LNS

Crystal structure of Asparagine synthetase A (AsnA) from Trypanosoma brucei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-07
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.976
Spacegroup name	P 61 2 2
Unit cell lengths	71.550, 71.550, 268.631
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.647 - 2.200
R-factor	0.2033
Rwork	0.201
R-free	0.24280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.169
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge		0.360
Number of reflections	21721
<I/σ(I)>		2.1
Completeness [%]	99.6	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	10% w/v PEG 20000, 20% v/v PEG MME 550, 0.03M of each NPS (sodium nitrate, disodium hydrogen phosphate, ammonium sulfate), 0.1M MOPS/HEPES-Na, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K