4LMZ
Structural insight into RNA recognition by RRM1+2 domain of human ETR-3 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-05-22 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 47.006, 70.134, 133.064 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.322 - 2.780 |
| R-factor | 0.2486 |
| Rwork | 0.244 |
| R-free | 0.29250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nmr |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.874 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CCP4 |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.322 | 2.850 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Number of reflections | 5715 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2 M NaCl, Tris-HCl, 35% (v/v) 2-methyl 2,4-pentandiol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
