4LMY

Structure of GAS PerR-Zn-Zn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-26
Detector	NOIR-1
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	33.109, 87.496, 59.389
Unit cell angles	90.00, 94.78, 90.00

Refinement procedure

Resolution	21.169 - 1.600
R-factor	0.2213
Rwork	0.220
R-free	0.23890
Structure solution method	MAD
RMSD bond length	0.003
RMSD bond angle	0.789
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	51675
Completeness [%]	95.6	79.7
Redundancy	4.8	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	298	0.1M Tris, 0.25M magnesium chloride, 27% PEG 4000, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	8.6	298	0.1M Tris, 0.25M magnesium chloride, 27% PEG 4000, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K