4LMW
Crystal structure of glutathione transferase GSTFuA3 from Phanerochaete chrysosporium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 87.000, 87.000, 225.703 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.164 - 2.099 |
| R-factor | 0.1883 |
| Rwork | 0.187 |
| R-free | 0.21590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g19 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.085 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.164 | 2.210 |
| High resolution limit [Å] | 2.099 | 2.100 |
| Rmerge | 0.064 | 0.490 |
| Number of reflections | 30431 | |
| <I/σ(I)> | 25.1 | 4 |
| Completeness [%] | 99.6 | 97.4 |
| Redundancy | 14.5 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 4.6 | 293 | 0.1M Sodium acetate, 2M Sodium formate, pH 4.6, microbatch under oil, temperature 293K |
