4LMT

Structure of The N-terminal domain of Coronavirus Nucleocapsid Protein complexed with NSC663284

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 65
Unit cell lengths	81.911, 81.911, 42.873
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.732 - 1.710
R-factor	0.2402
Rwork	0.236
R-free	0.27860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j3k
RMSD bond length	0.008
RMSD bond angle	1.373
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.770
High resolution limit [Å]	1.710	1.710
Number of reflections	17432
Completeness [%]	97.5	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	25% PEG1500, 0.25M SPG, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K