4LMS

Light harvesting complex PC645 from the cryptophyte Chroomonas sp. CCMP270

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-20
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.821
Spacegroup name	P 21 21 21
Unit cell lengths	44.958, 93.824, 121.908
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.038 - 1.350
R-factor	0.145
Rwork	0.143
R-free	0.18100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qgw
RMSD bond length	0.010
RMSD bond angle	1.333
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_833))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.800	1.420
High resolution limit [Å]	1.350	1.350
Rmerge	0.095	0.410
Number of reflections	109920
<I/σ(I)>	12.3	2.5
Completeness [%]	95.2	74.6
Redundancy	6.6	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	295	20% PEG4000, 20% isopropanol, 0.1 M sodium citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 295K