4LML
GLIC double mutant I9'A T25'A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-13 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 122.803, 127.997, 190.343 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.932 - 3.800 |
| R-factor | 0.2509 |
| Rwork | 0.246 |
| R-free | 0.30170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ehz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.222 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.120 | |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.230 | 1.239 |
| Number of reflections | 24202 | |
| <I/σ(I)> | 12 | 1.9 |
| Completeness [%] | 97.2 | 92 |
| Redundancy | 14 | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 10-12% PEG4000, 225 mM ammonium sulfate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
