4LMK
GLIC Liganded-closed-channel Conformation, Mutant Y27'A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.990, 134.280, 159.940 |
| Unit cell angles | 90.00, 102.06, 90.00 |
Refinement procedure
| Resolution | 48.761 - 3.220 |
| R-factor | 0.2121 |
| Rwork | 0.211 |
| R-free | 0.23830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ehz |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.378 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.270 | |
| High resolution limit [Å] | 3.220 | 3.220 |
| Rmerge | 0.206 | 1.268 |
| Number of reflections | 39396 | |
| <I/σ(I)> | 8.4 | 1.2 |
| Completeness [%] | 98.5 | 85.9 |
| Redundancy | 4.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 10-12% PEG4000, 225 mM ammonium sulfate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
