4LM6

Light harvesting complex PC612 from the cryptophyte Hemiselmis virescens M1635

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-29
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 63
Unit cell lengths	132.081, 132.081, 64.362
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.929 - 1.700
R-factor	0.1629
Rwork	0.161
R-free	0.19700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qgw CHAIN C
RMSD bond length	0.010
RMSD bond angle	1.316
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1415))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.130	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.078	0.790
Number of reflections	70433
<I/σ(I)>	24	3.3
Completeness [%]	98.3	98
Redundancy	11.3	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	25% PEG3350, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K