4LLH

Substrate bound outward-open state of the symporter BetP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Detector technology	PIXEL
Collection date	2012-04-17
Detector	PSI PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	115.470, 129.690, 164.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.300 - 2.800
R-factor	0.243
Rwork	0.240
R-free	0.28960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.740
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.330	12.500	3.040
High resolution limit [Å]	2.800	8.870	2.800
Rmerge	0.159	0.018	0.301
Number of reflections	48865
<I/σ(I)>	12.47	37.32	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	100 mM Na3citrate, 300 mM NaCl, 17-24% PEG400, 8 mM arseno-choline, VAPOR DIFFUSION, HANGING DROP, temperature 298K