4LL7
Structure of She3p amino terminus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1050 |
| Spacegroup name | P 1 |
| Unit cell lengths | 28.069, 49.576, 149.950 |
| Unit cell angles | 89.96, 84.67, 89.98 |
Refinement procedure
| Resolution | 29.860 - 2.310 |
| Rwork | 0.231 |
| R-free | 0.26440 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.142 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.310 |
| Number of reflections | 34442 |
| Completeness [%] | 97.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M MES, pH 6.5, 0.45M Ammonium sulfate, 10% (v/v) 2-propanol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
