4LKU
Structure of the C-terminal domain of the E. coli mechanosensitive channel of large conductance
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-01-09 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.250, 55.120, 32.850 |
Unit cell angles | 90.00, 93.58, 90.00 |
Refinement procedure
Resolution | 23.721 - 1.450 |
R-factor | 0.1717 |
Rwork | 0.169 |
R-free | 0.22510 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.022 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.21) |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 32.786 | 23.721 | 1.530 |
High resolution limit [Å] | 1.450 | 4.590 | 1.450 |
Rmerge | 0.095 | 0.742 | |
Total number of observations | 4336 | 16445 | |
Number of reflections | 19178 | ||
<I/σ(I)> | 6.2 | 4.1 | 0.9 |
Completeness [%] | 94.2 | 96.2 | 93.5 |
Redundancy | 5.9 | 6.7 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 0.1M sodium acetate pH 4.6, 0.34M ammonium acetate, 34% PEG 2000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 277K |