4LKP

Crystal Structure of Apo Human Epidermal Fatty Acid Binding Protein (FABP5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-04
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 43 21 2
Unit cell lengths	62.971, 62.971, 74.498
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.092 - 1.670
R-factor	0.1826
Rwork	0.181
R-free	0.21450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.825
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.670	3.600	1.670
Rmerge	0.042	0.024	0.389
Number of reflections	18056
<I/σ(I)>	21.1
Completeness [%]	99.9	99.9	99.4
Redundancy	9.1	8.8	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	291	2.0M ammonium sulfate, 300mM Na/K tartrate, 100mM Na citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K