4LKC

An Antibody Against the C-terminal Domain of PCSK9 lowers LDL Cholesterol Levels in vivo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-31
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0003
Spacegroup name	P 21 21 2
Unit cell lengths	92.062, 62.338, 84.014
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.570 - 2.200
R-factor	0.1752
Rwork	0.172
R-free	0.24510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.100
Refinement software	BUSTER (2.9.5)

Data quality characteristics

	Overall
Low resolution limit [Å]	92.060
High resolution limit [Å]	2.190
Number of reflections	25422

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	12.6% PEG 3350, 0.01 M spermine tetrahydrochloride, 0.0126 M trisodium citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K