4LKA

Crystal Structure of MOZ double PHD finger histone H3K9ac complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97372
Spacegroup name	P 43 21 2
Unit cell lengths	70.455, 70.455, 96.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.255 - 1.610
R-factor	0.1652
Rwork	0.164
R-free	0.18520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lk9
RMSD bond length	0.009
RMSD bond angle	1.170
Data reduction software	XDS
Data scaling software	SCALA (0.1.27)
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.190
High resolution limit [Å]	1.610
Rmerge	0.076
Number of reflections	32149
Completeness [%]	100.0
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	283	100 mM Na-Hepes, 1.4 M sodium citrate, pH 7.5, vapor diffusion, temperature 283K