4LK9

Crystal Structure of MOZ double PHD finger histone H3 tail complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8726
Spacegroup name	P 43 21 2
Unit cell lengths	70.519, 70.519, 96.827
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.864 - 1.600
R-factor	0.1647
Rwork	0.164
R-free	0.18630
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	1.648
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	57.003	49.864	1.690
High resolution limit [Å]	1.570	5.060	1.600
Rmerge		0.033	0.746
Total number of observations		5078	23467
Number of reflections	32766
<I/σ(I)>	14.3	19.1	1
Completeness [%]	99.7	98	99.7
Redundancy	4.9	4.4	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	283	100 mM Na-Hepes, 1.4 M sodium citrate, pH 7.5, VAPOR DIFFUSION, temperature 283K