4LK8
Crystal structure of CyaY protein from Psychromonas ingrahamii in complex with Co(II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-02 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.91907 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.891, 50.564, 46.310 |
| Unit cell angles | 90.00, 90.77, 90.00 |
Refinement procedure
| Resolution | 30.425 - 1.494 |
| R-factor | 0.1853 |
| Rwork | 0.184 |
| R-free | 0.21060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hs5 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.781 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.425 | 30.430 | 1.550 |
| High resolution limit [Å] | 1.494 | 3.230 | 1.500 |
| Number of reflections | 53634 | ||
| Completeness [%] | 92.0 | 87.1 | 77.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 297 | 20mM sodium acetate, 200mM MgCl2, 29.5% PEG 4000, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 297.0K |
