4LJS
The crystal structure of a periplasmic binding protein from Veillonella parvula DSM 2008
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 62 |
| Unit cell lengths | 82.994, 82.994, 110.419 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.119 - 2.321 |
| R-factor | 0.1564 |
| Rwork | 0.154 |
| R-free | 0.20770 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.915 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.200 | 2.360 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.102 | 0.571 |
| Number of reflections | 18692 | |
| <I/σ(I)> | 38.3 | 5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.5 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 297 | 0.8M Potassium sodium tartrate, 0.1M Tris:HCl, 0.5%(w/v) PEG MME 5000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
