4LJL
Structural insights into the unique single-stranded DNA binding mode of DNA processing protein A from Helicobacter pylori
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 171.090, 107.860, 37.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.760 - 2.200 |
R-factor | 0.1718 |
Rwork | 0.170 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ljk |
RMSD bond length | 0.006 |
RMSD bond angle | 0.976 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 45.620 |
High resolution limit [Å] | 2.200 |
Number of reflections | 34651 |
Completeness [%] | 95.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 25% PEG3350, 100 mM Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |