4LJK
Structural insights into the unique single-stranded DNA binding mode of DNA processing protein A from Helicobacter pylori
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-25 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 117.880, 42.430, 181.330 |
Unit cell angles | 90.00, 108.65, 90.00 |
Refinement procedure
Resolution | 59.227 - 2.354 |
R-factor | 0.1897 |
Rwork | 0.186 |
R-free | 0.25370 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.058 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 59.510 |
High resolution limit [Å] | 2.350 |
Number of reflections | 70980 |
Completeness [%] | 99.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 25% PEG3350, 100 mM HEPES, 100 mM potassium thiocyanate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |