4LJD

Structure of a photobleached state of IrisFP under low intensity laser-light

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99979
Spacegroup name	P 21 21 21
Unit cell lengths	85.906, 96.487, 140.136
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.616 - 2.500
R-factor	0.2022
Rwork	0.199
R-free	0.25590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.930
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.340	2.640
High resolution limit [Å]	2.500	2.500
Number of reflections	40184
<I/σ(I)>	11.8
Completeness [%]	97.8	97.5
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	2.1M ammonium sulfate, 0.1M bicine, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K