4LJC
Structure of an X-ray-induced photobleached state of IrisFP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-06-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.972 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.102, 96.636, 139.711 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.869 - 1.860 |
R-factor | 0.2037 |
Rwork | 0.202 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2VVH - IRISFP FLUORESCENT PROTEIN IN ITS GREEN FORM CIS CONFORMATION |
RMSD bond length | 0.012 |
RMSD bond angle | 1.631 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.410 | 1.960 |
High resolution limit [Å] | 1.860 | 1.860 |
Number of reflections | 99096 | |
<I/σ(I)> | 10.4 | |
Completeness [%] | 99.3 | 98.6 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 2.1M ammonium sulfate, 0.1M bicine, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |