4LJ6
ClpB NBD2 from T. thermophilus in complex with AMPPCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2006-04-07 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 65 |
| Unit cell lengths | 74.050, 74.050, 118.670 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.21923 |
| Rwork | 0.217 |
| R-free | 0.26236 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lj5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.227 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.074 | 0.554 |
| Number of reflections | 28959 | |
| <I/σ(I)> | 14.1 | 3.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2M sodium citrate, 0.1M HEPES/NaOH, 20% isopropanol, 2mM AMPPCP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
