4LJ5
ClpB NBD2 from T. thermophilus in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.01096 |
| Spacegroup name | P 65 |
| Unit cell lengths | 76.060, 76.060, 120.540 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.270 - 2.400 |
| R-factor | 0.23706 |
| Rwork | 0.235 |
| R-free | 0.27211 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qvr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.922 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.085 | 0.774 |
| Number of reflections | 17302 | |
| <I/σ(I)> | 10.12 | 2.12 |
| Completeness [%] | 98.4 | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2M sodium citrate, 0.1M HEPES/NaOH, 20% isopropanol, 2mM ADP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
