4LJ5
ClpB NBD2 from T. thermophilus in complex with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.01096 |
Spacegroup name | P 65 |
Unit cell lengths | 76.060, 76.060, 120.540 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.270 - 2.400 |
R-factor | 0.23706 |
Rwork | 0.235 |
R-free | 0.27211 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qvr |
RMSD bond length | 0.006 |
RMSD bond angle | 0.922 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.085 | 0.774 |
Number of reflections | 17302 | |
<I/σ(I)> | 10.12 | 2.12 |
Completeness [%] | 98.4 | 98.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2M sodium citrate, 0.1M HEPES/NaOH, 20% isopropanol, 2mM ADP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |