4LIR

Crystal structure of a nucleoporin 35kDa (NUP35) from Homo sapiens at 2.46 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-12
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97949,0.97929
Spacegroup name	P 65 2 2
Unit cell lengths	70.590, 70.590, 160.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.558 - 2.460
R-factor	0.1915
Rwork	0.191
R-free	0.20580
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	0.990
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.558	28.558	2.520
High resolution limit [Å]	2.460	11.000	2.460
Rmerge		0.047	0.982
Total number of observations		645	4738
Number of reflections	9217
<I/σ(I)>	12.4	11.9	0.8
Completeness [%]	99.9	93.7	100
Redundancy	6.8	4.7	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	277	20.0% MPD, 0.1M Citrate pH 4.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K