4LIJ

Crystal structure of a far upstream element (FUSE) binding protein 1 (FUBP1) from Homo sapiens at 1.95 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9537,0.9796,0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	86.810, 86.810, 84.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.384 - 1.800
R-factor	0.173
Rwork	0.172
R-free	0.19920
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.553
Data reduction software	XDS
Data scaling software	XSCALE (July 4, 2012)
Phasing software	SOLVE
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	61.384	61.384	1.850
High resolution limit [Å]	1.800	8.050	1.800
Rmerge	0.143	0.030	0.923
Number of reflections	24100	634	4174
<I/σ(I)>	9.17	29.7	1.6
Completeness [%]	78.6	99.8	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	20.00% Glycerol, 1.60M ammonium dihydrogen phosphate, 0.1M TRIS pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K