4LII
Crystal structure of an apoptosis-inducing factor, mitochondrion-associated, 1 (AIFM1) from Homo sapiens at 1.88 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-29 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.680, 89.807, 60.471 |
Unit cell angles | 90.00, 94.78, 90.00 |
Refinement procedure
Resolution | 44.903 - 1.880 |
R-factor | 0.169 |
Rwork | 0.167 |
R-free | 0.21120 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.489 |
Data reduction software | XDS |
Data scaling software | XSCALE (December 29, 2011) |
Phasing software | SHELX |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.903 | 44.903 | 1.950 |
High resolution limit [Å] | 1.880 | 4.040 | 1.880 |
Rmerge | 0.112 | 0.051 | 0.872 |
Number of reflections | 42757 | 4310 | 4415 |
<I/σ(I)> | 7.73 | 19.2 | 1.6 |
Completeness [%] | 97.4 | 95.3 | 97.4 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 20.00% polyethylene glycol 6000, 0.1M MES pH 6.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |