4LIH
The crystal structure of Gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase from Burkholderia cenocepacia J2315
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-04-17 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 1 |
| Unit cell lengths | 90.310, 104.190, 108.280 |
| Unit cell angles | 91.70, 99.04, 90.02 |
Refinement procedure
| Resolution | 48.190 - 1.850 |
| R-factor | 0.19097 |
| Rwork | 0.189 |
| R-free | 0.23175 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fr8 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.513 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.107 | 0.548 |
| Number of reflections | 325553 | |
| <I/σ(I)> | 13.3 | 3.52 |
| Completeness [%] | 97.8 | 96.6 |
| Redundancy | 5.8 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | Morpheus Screen well E2: 0.12 Ethylene glycols, 0.1M buffer 1, 30% ethylene glycol/PEG8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
