4LI2
Crystal Structures of Lgr4 and its complex with R-spondin1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-08 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 103.379, 160.923, 82.216 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.613 - 3.190 |
R-factor | 0.2347 |
Rwork | 0.229 |
R-free | 0.28360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4li1 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.310 |
High resolution limit [Å] | 3.200 | 6.890 | 3.200 |
Rmerge | 0.087 | 0.036 | 0.966 |
Number of reflections | 11577 | ||
<I/σ(I)> | 5.3 | ||
Completeness [%] | 98.5 | 96.7 | 99 |
Redundancy | 4.4 | 4.3 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.7 | 298 | 22% PEG3350, 200 mM MgCl2 and 100 mM BisTris pH 5.7, vapor diffusion, temperature 298K |