4LHX
Crystal structure of nucleotide-free Rab8:Rabin8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 81.645, 165.301, 166.404 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.940 - 3.050 |
R-factor | 0.26537 |
Rwork | 0.264 |
R-free | 0.29996 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yzq |
RMSD bond length | 0.018 |
RMSD bond angle | 1.688 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.900 |
High resolution limit [Å] | 3.050 |
Number of reflections | 21696 |
Completeness [%] | 99.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 18% (w/v) PEG3350, 0.1 M Li2SO4, 0.1 M MES. cryo solution (30% (w/v) PEG3350, 0.1 M Li2SO4, 0.1 M MES), pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |