4LHL
Structure of the N-terminal domain of the Flo1 adhesin (N-Flo1p) from the yeast Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.800, 61.770, 106.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.380 - 1.430 |
| R-factor | 0.13417 |
| Rwork | 0.132 |
| R-free | 0.16527 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xjq |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.026 |
| Data reduction software | xia2 (automated system) |
| Data scaling software | xia2 (automated system) |
| Phasing software | BALBES (online server) |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.390 | 1.470 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.085 | 0.647 |
| Number of reflections | 56735 | |
| <I/σ(I)> | 13.6 | 3 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 7.2 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | Protein solution: 50 mM sodium acetate pH 5, 150 mM NaCl, 10 mM CaCl2 Crystallization condition: 2 M NaCl, 10% v/v EtOH, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
