4LHE
Crystal structure of closed form of Monoacylglycerol Lipase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-10 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 99.755, 106.191, 43.053 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.342 - 1.962 |
| R-factor | 0.2451 |
| Rwork | 0.243 |
| R-free | 0.28020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r1d |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.314 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP (11.0) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.342 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.118 | 0.617 |
| Number of reflections | 33489 | |
| <I/σ(I)> | 23.9 | 3.7 |
| Completeness [%] | 99.7 | 98.1 |
| Redundancy | 6.9 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 277 | 1.5M ammonium sulphate, 100mM citrate, 0.17M tartrate, 20% benzamidine, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
