4LHD
Crystal structure of Synechocystis sp. PCC 6803 glycine decarboxylase (P-protein), holo form with pyridoxal-5'-phosphate and glycine, closed flexible loop
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9334 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 159.992, 159.992, 159.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.762 - 1.796 |
| R-factor | 0.1639 |
| Rwork | 0.163 |
| R-free | 0.19140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lhc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.097 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.860 |
| High resolution limit [Å] | 1.796 | 1.796 |
| Rmerge | 0.094 | 0.471 |
| Number of reflections | 217331 | |
| <I/σ(I)> | 16.6 | 3.3 |
| Completeness [%] | 99.3 | 95.4 |
| Redundancy | 4.6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 100 mM Bicine pH 9, 1.6 M (NH4)3PO4, 5 mM TCEP and 0.2 mM PLP, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
