4LHC
Crystal structure of Synechocystis sp. PCC 6803 glycine decarboxylase (P-protein), holo form with pyridoxal-5'-phosphate and glycine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9334 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 160.050, 160.050, 159.833 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.783 - 1.899 |
| R-factor | 0.1562 |
| Rwork | 0.155 |
| R-free | 0.18560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lgl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.076 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.970 |
| High resolution limit [Å] | 1.899 | 1.899 |
| Rmerge | 0.068 | 0.371 |
| Number of reflections | 185454 | |
| <I/σ(I)> | 18.4 | 4.3 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 5.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 293 | 100 mM Bicine pH 9, 1.6 M (NH4)3PO4, 5 mM TCEP and 0.2 mM PLP, VAPOR DIFFUSION, temperature 293K |
