4LH2
Structure of mouse 1-Pyrroline-5-Carboxylate Dehydrogenase (ALDH4A1) complexed with succinate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 148 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-08-24 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.884, 94.091, 132.339 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.677 - 1.673 |
| R-factor | 0.1649 |
| Rwork | 0.164 |
| R-free | 0.18880 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4v9j |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.032 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.677 | 19.677 | 1.760 |
| High resolution limit [Å] | 1.673 | 5.290 | 1.670 |
| Rmerge | 0.032 | 0.342 | |
| Total number of observations | 20341 | 54933 | |
| Number of reflections | 118798 | ||
| <I/σ(I)> | 16.3 | 16.7 | 2.2 |
| Completeness [%] | 97.0 | 98 | 87.9 |
| Redundancy | 4.6 | 5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 15-25% PEG 3350, 0.2 M lithium sulfate, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
