4LGS
Ricin A chain bound to camelid nanobody (VHH4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.08 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.391, 49.935, 114.442 |
| Unit cell angles | 90.00, 103.88, 90.00 |
Refinement procedure
| Resolution | 44.361 - 2.700 |
| R-factor | 0.2024 |
| Rwork | 0.200 |
| R-free | 0.24780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.858 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.450 |
| High resolution limit [Å] | 2.410 | 6.540 | 2.410 |
| Rmerge | 0.093 | 0.044 | 0.739 |
| Number of reflections | 17642 | ||
| <I/σ(I)> | 10.3 | ||
| Completeness [%] | 90.1 | 97.2 | 53 |
| Redundancy | 3.1 | 3.3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 100 mM NaHepes, 20% PEG 8000, pH 7.5, VAPOR DIFFUSION, temperature 293K |
