4LGN
The structure of Acidothermus cellulolyticus family 74 glycoside hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-05 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.761, 78.686, 143.952 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.410 - 1.820 |
| R-factor | 0.1525 |
| Rwork | 0.150 |
| R-free | 0.19640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cn3 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.931 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.410 | 1.910 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Number of reflections | 76875 | |
| <I/σ(I)> | 8.52 | 1.98 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.66 | 4.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 294 | 0.1 M tri-Sodium citrate Dihydrate pH 5.0, 1.5 M Na Formate, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
