4LGM
Crystal Structure of Sulfolobus solfataricus Vps4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 64 |
| Unit cell lengths | 101.321, 101.321, 64.743 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.166 - 2.711 |
| R-factor | 0.2208 |
| Rwork | 0.217 |
| R-free | 0.25510 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.003 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.13) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.830 |
| High resolution limit [Å] | 2.711 | 5.870 | 2.711 |
| Rmerge | 0.073 | 0.049 | 0.657 |
| Number of reflections | 19379 | ||
| <I/σ(I)> | 26.5 | ||
| Completeness [%] | 99.7 | 97 | 99.9 |
| Redundancy | 5.9 | 5.7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 70 % MPD, 100 mM HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
