4LGM
Crystal Structure of Sulfolobus solfataricus Vps4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97945 |
Spacegroup name | P 64 |
Unit cell lengths | 101.321, 101.321, 64.743 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.166 - 2.711 |
R-factor | 0.2208 |
Rwork | 0.217 |
R-free | 0.25510 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.003 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.13) |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.830 |
High resolution limit [Å] | 2.711 | 5.870 | 2.711 |
Rmerge | 0.073 | 0.049 | 0.657 |
Number of reflections | 19379 | ||
<I/σ(I)> | 26.5 | ||
Completeness [%] | 99.7 | 97 | 99.9 |
Redundancy | 5.9 | 5.7 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 70 % MPD, 100 mM HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |