4LGL
Crystal Structure of Glycine Decarboxylase P-protein from Synechocystis sp. PCC 6803, apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.299, 135.814, 179.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.107 - 2.000 |
| R-factor | 0.1622 |
| Rwork | 0.161 |
| R-free | 0.19190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1WYT heterodimer |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.058 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.110 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.083 | 0.555 |
| Number of reflections | 158314 | |
| <I/σ(I)> | 26.88 | 3.53 |
| Completeness [%] | 98.8 | 100 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.75 | 293 | 100 mM Tris-HCl pH 7.75, 17% PEG 3350, 0.25 M LiCl and 10 mM 2-mercaptoethanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
